ETS2 (Center) Rabbit Polyclonal Antibody

CAT#: AP51471PU-N

ETS2 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of ETS2 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). ETS2 (arrow) was detected using the purified Pab.

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  Formalin fixed and paraffin embedded brain tissue reacted with ETS2 Antibody (Center) followed by peroxidase conjugation of the secondary antibody and DAB staining.

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  Flow cytometric analysis of MCF-7 cells using ETS2 Antibody (Center) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 152~182 amino acids from the Central region of Human ETS2.
• Specificity: This antibody recognizes Human ETS2 (Center).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens ETS proto-oncogene 2, transcription factor (ETS2), transcript variant 1
• Database Link:
  Entrez Gene 2114 Human
• Background: ETS transcriptions factors, such as ETS2, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes.
• Synonyms: Protein C-ets-2
• Note: Molecular Weight: 53001 Da

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Dorso-ventral axis formation