Factor XII (F12) (N-term) Rabbit Polyclonal Antibody

CAT#: AP51493PU-N

Factor XII (F12) (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of F12 Antibody (N-term) in K562, CEM, MDA-MB435 cell line lysates (35ug/lane). This demonstrates the F12 antibody detected the F12 protein (arrow).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 13~43 amino acids from the N-terminal region of human F12
• Specificity: This antibody recognizes Human Coagulation factor XII (F12) (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens coagulation factor XII (F12)
• Database Link:
  Entrez Gene 2161 Human
• Background: This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI.
• Synonyms: Hageman factor, HAF
• Note: Molecular Weight: 67818 Da

Reference Data

• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades