FUS (C-term) Rabbit Polyclonal Antibody

CAT#: AP51735PU-N

FUS (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of FUS Antibody (C-term) in mouse cerebellum tissue lysates (15ug/lane).This demonstrates the FUS antibody detected FUS protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma stained with FUS Antibody (C-term) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FUS antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  Confocal immunofluorescent analysis of FUS Antibody (C-term) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

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  Flow cytometric analysis of Hela cells using FUS Antibody (C-term) (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunofluorescence: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 506-534 amino acids from the C-terminal region of human FUS
• Specificity: This antibody recognizes Human and Mouse FUS (C-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens FUS RNA binding protein (FUS), transcript variant 3
• Database Link:
  Entrez Gene 233908 MouseEntrez Gene 2521 Human
• Background: This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6.
• Synonyms: POMp75
• Note: Molecular Weight: 53426 Da

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency