FUT4 (C-term) Rabbit Polyclonal Antibody

CAT#: AP51738PU-N

FUT4 (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of FUT4 Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). This demonstrates the FUT4 antibody detected the FUT4 protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue reacted with FUT4 Antibody (C-term) followed by peroxidase conjugation of the secondary antibody and DAB staining.

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  Flow cytometric analysis of NCI-H460 cells using FUT4 Antibody (C-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 502-530 amino acids from the C-terminal region of Human Fucosyltransferase 4
• Specificity: This antibody recognizes Human and Mouse Fucosyltransferase 4 (C-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific) (FUT4)
• Database Link:
  Entrez Gene 2526 Human
• Background: The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15).
• Synonyms: Fucosyltransferase IV, FUT4, ELFT, FCT3A, Fuc-TIV
• Note: Molecular Weight: 59084 Da

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - lacto and neolacto series, Metabolic pathways