GALNT3 (Center) Rabbit Polyclonal Antibody

CAT#: AP51768PU-N

GALNT3 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of GALNT3 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). GALNT3 (arrow) was detected using the purified Pab.

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  Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human skin tissue reacted with GALNT3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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  Flow cytometric analysis of MCF-7 cells using GALNT3 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 461~490 amino acids from the Center region of human GALNT3
• Specificity: This antibody recognizes Human GALNT3 (Center).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: polypeptide N-acetylgalactosaminyltransferase 3
• Database Link:
  Entrez Gene 2591 Human
  UniProt ID Q14435
• Background: GALNT3 encodes UDP-GalNAc transferase 3, a member of the GalNAc-transferases family. This family transfers an N-acetyl galactosamine to the hydroxyl group of a serine or threonine residue in the first step of O-linked oligosaccharide biosynthesis. Individual GalNAc-transferases have distinct activities and initiation of O-glycosylation is regulated by a repertoire of GalNAc-transferases. The protein is highly homologous to other family members, however the enzymes have different substrate specificities.
• Synonyms: GalNAc transferase 3, pp-GaNTase 3, GalNAc-T3
• Note: Molecular Weight: 72610 Da

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis