GBA3 (C-term) Rabbit Polyclonal Antibody

CAT#: AP51789PU-N

GBA3 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Cytosolic beta-glucosidase Antibody (C-term) in NCI-H292 cell line lysates (35ug/lane). This demonstrates the GBA3 antibody detected the GBA3 protein (arrow).

• 

  Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue reacted with Cytosolic beta-glucosidase Antibody (C-term) followed by peroxidase conjugation of the secondary antibody and DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 295-325 amino acids from the C-terminal region of Human Cytosolic beta-glucosidase
• Specificity: This antibody recognizes Human Cytosolic beta-glucosidase (C-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens glucosylceramidase beta 3 (gene/pseudogene) (GBA3), transcript variant 2, coding
• Database Link:
  Entrez Gene 57733 Human
• Background:
  GBA3, or cytosolic beta-glucosidase (EC 3.2.1.21), is a predominantly liver enzyme that efficiently hydrolyzes beta-D-glucoside and beta-D-galactoside, but not any known physiologic beta-glycoside, suggesting that it may be involved in detoxification of plant glycosides (de Graaf et al., 2001 [PubMed 11389701]). GBA3 also has significant neutral glycosylceramidase activity (EC 3.2.1.62), suggesting that it may be involved in a nonlysosomal catabolic pathway of glucosylceramide metabolism (Hayashi et al., 2007 [PubMed 17595169]).
• Synonyms: GBA3, CBG, CBGL1
• Note: Molecular Weight: 53696 Da

Reference Data

• Protein Pathways: Cyanoamino acid metabolism, Starch and sucrose metabolism