GPC1 (N-term) Rabbit Polyclonal Antibody

CAT#: AP51865PU-N

GPC1 (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of Glypican-1 / GPC1Antibody (N-term) in Hela cell line lysates (35ug/lane). Glypican-1 (arrow) was detected using the purified Pab.

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  Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human lung carcinoma reacted with Glypican-1 / GPC1Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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  Flow cytometric analysis of Hela cells using Glypican-1 / GPC1Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 12~41 amino acids from the N-terminal region of human Glypican-1 / GPC1
• Specificity: This antibody recognizes Human Glypican-1 / GPC1 (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens glypican 1 (GPC1)
• Database Link:
  Entrez Gene 2817 Human
• Background:
  Glypican-1 (GPC1) is a cell surface proteoglycan that contains heparan sulphate. The protein is attached to the cell membrane by a GPI anchor. GPC1 is required for efficient TGF-beta1 signalling in pancreatic cancer cells. Members of the glypican-related integral membrane proteoglycan family (GRIPS) may play a role in the control of cell division and growth regulation.
• Synonyms: FLJ38078; glypican
• Note: Molecular Weight: 61680 Da

Reference Data

• Protein Families: Druggable Genome