GLCNE (GNE) (N-term) Rabbit Polyclonal Antibody

CAT#: AP51881PU-N

GLCNE (GNE) (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of GNE / GLCNE Antibody (N-term) in human placenta tissue lysates (35ug/lane). This demonstrates the GNE antibody detected the GNE protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue reacted with GNE / GLCNE Antibody (N-term) followed by peroxidase conjugation of the secondary antibody and DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 168-197 amino acids from the N-terminal region of Human GNE / GLCNE
• Specificity: This antibody recognizes Human GNE / GLCNE (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant 1
• Database Link:
  Entrez Gene 10020 Human
• Background: The protein encoded by this gene is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells. Mutations in this gene are associated with sialuria, autosomal recessive inclusion body myopathy, and Nonaka myopathy. Alternative splicing of this gene results in transcript variants encoding different isoforms. [provided by RefSeq].
• Synonyms: UDP-GlcNAc-2-epimerase/ManAc kinase
• Note: Molecular Weight: 79275 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Metabolic pathways