Glutathione Peroxidase 1 (GPX1) (C-term) Rabbit Polyclonal Antibody

CAT#: AP51937PU-N

Glutathione Peroxidase 1 (GPX1) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Glutathione peroxidase 1 / GPX1 (arrow) in mouse liver tissue lysates (35ug/lane) using Glutathione peroxidase 1 / GPX1 antibody. (C-term).

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  Immunohistochemistry analysis of human hepatocarcinoma tissue (Formalin-fixed, Paraffin-embedded) using Glutathione peroxidase 1 / GPX1 antibody. (C-term), followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of this antibody for IHC; clinical relevance has not been evaluated.

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  Confocal immunofluorescent analysis of GPX1 in HepG2 cells using Glutathione peroxidase 1 / GPX1 antibody. (C-term), followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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  Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram) using Glutathione peroxidase 1 / GPX1 antibody. (C-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodies.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution:

  Enzyme immunoassay: 1/1000.
  Flow cytometry: 1/10-1/50.
  Immunofluorescence: 1/10-1/50.
  Immunohistochemistry on Paraffin sections: 1/10-1/50.
  Western blot: 1/1000.
  
  

• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 164-193aa) of human Glutathione peroxidase 1 / GPX1.
• Specificity: This antibody recognizes Glutathione peroxidase 1 / GPX1.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: glutathione peroxidase 1
• Database Link:
  Entrez Gene 14775 MouseEntrez Gene 2876 Human
  UniProt ID P07203
• Background: GPX1 encodes a member of the glutathione peroxidase family: Glutathione peroxidase 1. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence.
• Synonyms: GSHPx-1, Cellular glutathione peroxidase
• Note: Molecular Weight: 21946 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Arachidonic acid metabolism, Glutathione metabolism, Huntington's disease