HADHB (C-term) Rabbit Polyclonal Antibody

CAT#: AP51996PU-N

HADHB (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of HADHB Antibody (C-term) in Jurkat cell line lysates (35ug/lane). HADHB (arrow) was detected using the purified Pab.

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  Flow cytometric analysis of Jurkat cells using HADHB Antibody (C-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 321~351 amino acids from the C-terminal region of human HADHB
• Specificity: This antibody recognizes Human HADHB (C-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit (HADHB), transcript variant 1
• Database Link:
  Entrez Gene 3032 Human
• Background: HADHB encodes the beta subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the beta subunit catalyzing the 3-ketoacyl-CoA thiolase activity. Mutations in this gene result in trifunctional protein deficiency. The encoded protein can also bind RNA and decreases the stability of some mRNAs. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation.
• Synonyms: MSTP029, TP-beta, 3-ketoacyl-CoA thiolase
• Note: Molecular Weight: 51294 Da

Reference Data

• Protein Pathways: Fatty acid elongation in mitochondria, Fatty acid metabolism, Metabolic pathways, Valine, leucine and isoleucine degradation