Repulsive Guidance Molecule C (HFE2) (C-term) Rabbit Polyclonal Antibody

CAT#: AP52037PU-N

Repulsive Guidance Molecule C (HFE2) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of HFE2 / Hemojuvelin Antibody (C-term) in HepG2 cell line lysates (35ug/lane). HFE2 (arrow) was detected using the purified Pab.

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  Flow cytometric analysis of HepG2 cells using HFE2 / Hemojuvelin Antibody (C-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This HFE2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 308-338 amino acids from the C-terminal region of human HFE2.
• Specificity: This antibody recognizes Human HFE2 / Hemojuvelin (C-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens hemochromatosis type 2 (juvenile) (HFE2), transcript variant b
• Database Link:
  Entrez Gene 148738 Human
• Background: HFE2 is involved in iron metabolism. It may be a component of the signaling pathway which activates hepcidin or it may act as a modulator of hepcidin expression. It could also represent the cellular receptor for hepcidin. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Defects in this gene are the cause of hemochromatosis type 2A, also called juvenile hemochromatosis (JH). JH is an early-onset autosomal recessive disorder due to severe iron overload resulting in hypogonadotrophic hypogonadism, hepatic fibrosis or cirrhosis and cardiomyopathy, occurring typically before age of 30.
• Synonyms: HJV, RGMC
• Note: Molecular Weight: 45.08 kDa

Reference Data

• Protein Families: Transmembrane