HLA-DRB5 (Center) Rabbit Polyclonal Antibody

CAT#: AP52055PU-N

HLA-DRB5 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of HLA-DRB5 Antibody (Center) in HL-60 cell line lysates (35ug/lane). HLA-DRB5 (arrow) was detected using the purified Pab.

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma reacted with HLA-DRB5 Antibody (Center), which was peroxidase conjugated to the secondary antibody and followed by DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution:

  Western Blot: 1/100-1/500.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 43~70 amino acids from the Central region of human HLA class II DRB5 beta / HLA-DRB5
• Specificity: This antibody recognizes Human HLA class II DRB5 beta / HLA-DRB5 (Center).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens major histocompatibility complex, class II, DR51 haplotype (DR51) on chromosome 6
• Database Link:
  Entrez Gene 3127 Human
• Background: HLA-DRB5 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB5 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.
• Synonyms: DR beta-5, DR2-beta-2, Dw2, MHC class II antigen DRB5
• Note: Molecular Weight: 30056 Da

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Hematopoietic cell lineage, Systemic lupus erythematosus, Type I diabetes mellitus, Viral myocarditis