ID1 (Center) Rabbit Polyclonal Antibody

CAT#: AP52144PU-N

ID1 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of ID1 / BHLHB24 Antibody (Center) in U251 cell line lysates (35ug/lane). This demonstrates the ID1 antibody detected the ID1 protein (arrow).

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  Western blot analysis of ID1 / BHLHB24 Antibody (Center) in mouse heart tissue lysates (35ug/lane). This demonstrates the ID1 (PEI 1:100) antibody detected the ID1 protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue reacted with ID1 / BHLHB24 Antibody (Center), which was peroxidase conjugated to the secondary antibody and followed by DAB staining.

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  Confocal immunofluorescent analysis of ID1 / BHLHB24 Antibody (Center) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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  Flow cytometric analysis of U251 cells using ID1 / BHLHB24 Antibody (Center) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunofluorescence: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 73-101 amino acids from the Central region of Human ID1 / BHLHB24
• Specificity: This antibody recognizes Human and Mouse ID1 / BHLHB24 (Center).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens inhibitor of DNA binding 1, HLH protein (ID1), transcript variant 1
• Database Link:
  Entrez Gene 15901 MouseEntrez Gene 3397 Human
• Background:
  The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
• Synonyms: DNA-binding protein inhibitor ID-1, Inhibitor of DNA binding 1
• Note: Molecular Weight: 16133 Da

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: TGF-beta signaling pathway