IEX1 (IER3) (N-term) Rabbit Polyclonal Antibody

CAT#: AP52148PU-N

IEX1 (IER3) (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of IER3 / IEX1 Antibody (N-term) in mouse heart tissue lysates (35ug/lane). This demonstrates the IER3 antibody detected the IER3 protein (arrow).

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  Western blot analysisof IER3 / IEX1 Antibody (N-term) in A549 cell line lysates (35ug/lane). This demonstrates the IER3 antibody detected the IER3 protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue reacted with IER3 / IEX1 Antibody (N-term), which was peroxidase conjugated to the secondary antibody and followed by AB staining.

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  Confocal immunofluorescent analysis of IER3 / IEX1 Antibody (N-term) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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  Flow cytometric analysis of A549 cells using IER3 / IEX1 Antibody (N-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: Peptide ELISA: 1/1000.
  Western Blot: 1/1000.
  Flow Cytometry: 1/10-1/50.
  Immunofluorescence: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 38-65 amino acids from the N-terminal region of human IER3 / IEX1
• Specificity: This antibody recognizes Human and Mouse IER3 / IEX1 (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens immediate early response 3 (IER3)
• Database Link:
  Entrez Gene 15937 MouseEntrez Gene 8870 Human
• Background: This gene functions in the protection of cells from Fas- or tumor necrosis factor type alpha-induced apoptosis. Partially degraded and unspliced transcripts are found after virus infection in vitro, but these transcripts are not found in vivo and do not generate a valid protein.
• Synonyms: DIF2, IEX-1, PRG1, GLY96
• Note: Molecular Weight: 16929 Da

Reference Data

• Protein Families: Druggable Genome