Ikaros (IKZF1) (C-term) Rabbit Polyclonal Antibody

CAT#: AP52180PU-N

Ikaros (IKZF1) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in CEM cell line lysates (35ug/lane) using IKZF1/IKAROS antibody. (C-term). This demonstrates the IKZF1 antibody detected the IKZF1 protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue using IKZF1/IKAROS antibody. (C-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IKZF1 antibody for IHC. Clinical relevance has not been evaluated.

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  Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram) using IKZF1/IKAROS antibody. (C-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodies.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: This antibody is suitable for flow cytometry, immunohistochemistry on paraffin sections and Western blotting.
  The suggested dilution is:
  Flow cytometry: 1/10-1/50.
  Immunohistochemistry on paraffin sections: 1/50-1/100.
  Western blotting: 1/100-1/500.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 422-452aa) of human IKZF1.
• Specificity: This antibody detects human IKZF1 at c-term.
• Formulation: PBS with 0.09% (W/V) Sodium Azide.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a protein A column; followed by peptide affinity purification.
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens IKAROS family zinc finger 1 (IKZF1), transcript variant 2
• Database Link:
  Entrez Gene 10320 Human
• Background: Ikaros functions in the specification and the maturation of the T lymphocyte. It also interacts with a critical control element in the terminal deoxynucleotidyltransferase promoter as well as with the promoters for other genes expressed during early stages of B and T cell development.
• Synonyms: IK1, LYF-1, LYF1, ZNFN1A1
• Note: Molecular Weight: 57528 Da

Reference Data

• Protein Families: Druggable Genome, Transcription Factors