KDM4B Rabbit Polyclonal Antibody

CAT#: AP52270PU-N

KDM4B rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Phospho-JMJD2B-pT305 in MDA-MB435 cell line lysates (35ug/lane). JMJD2B (arrow) was detected using the purified Pab.

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  Western blot analysis of Phospho-JMJD2B-pT305 in NIH-3T3 cell line lysates (35ug/lane). JMJD2B (arrow) was detected using the purified Pab.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 287~316 amino acids from the C-terminal region of human JMJD2B
• Specificity: This antibody recognizes Human and Mouse JMJD2B (Thr305)
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens lysine demethylase 4B (KDM4B)
• Database Link:
  Entrez Gene 193796 MouseEntrez Gene 23030 Human
• Background:
  Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate.
• Synonyms: JHDM3B, KIAA0876
• Note: Molecular Weight: 121897 Da

Reference Data

• Protein Families: Druggable Genome