Kininogen 1 (KNG1) (N-term) Rabbit Polyclonal Antibody

CAT#: AP52400PU-N

Kininogen 1 (KNG1) (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis using Kininogen-1 Antibody (N-term) in Ramos cell line lysates (35ug/lane). This demonstrates the KNG1 antibody detected the KNG1 protein (arrow).

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  Western blot analysis of KNG1 (arrow) using Kininogen-1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KNG1 gene.

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma reacted with Kininogen-1 Antibody (N-term), which was peroxidase conjugated to the secondary antibody and followed by DAB staining.

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  Flow cytometric analysis of Ramos cells using Kininogen-1 Antibody (N-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 145-174 amino acids from the N-terminal region of Human Kininogen-1
• Specificity: This antibody recognizes Human Kininogen-1 (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens kininogen 1 (KNG1), transcript variant 2
• Database Link:
  Entrez Gene 3827 Human
• Background: This gene uses alternative splicing to generate two different proteins- high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. In contrast to HMWK, LMWK is not involved in blood coagulation. Three transcript variants encoding different isoforms have been found for this gene.
• Synonyms: Fitzgerald factor, KNG1, BDK, KNG
• Note: Molecular Weight: 71957 Da

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Complement and coagulation cascades