Leptin (LEP) (N-term) Rabbit Polyclonal Antibody

CAT#: AP52465PU-N

Leptin (LEP) (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Leptin Antibody (N-term) in Hela cell line lysates (35ug/lane).This demonstrates the LEP antibody detected the LEP protein (arrow).

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  Western blot analysis of LEP (arrow) using Leptin Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LEP gene.

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue reacted with Leptin Antibody (N-term) followed by peroxidase conjugation of the secondary antibody and DAB staining.

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  Confocal immunofluorescent analysis of Leptin Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

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  Flow cytometric analysis of Hela cells using Leptin Antibody (N-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunofluorescence: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 7-37 amino acids from the N-terminal region of Human Leptin.
• Specificity: This antibody recognizes Human Leptin (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens leptin (LEP)
• Database Link:
  Entrez Gene 3952 Human
• Background: This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq].
• Synonyms: LEP, OB, OBS, Obesity factor, Obese protein
• Note: Molecular Weight: 18641 Da

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Adipocytokine signaling pathway, Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, Neuroactive ligand-receptor interaction