MCM2 (C-term) Rabbit Polyclonal Antibody

CAT#: AP52637PU-N

MCM2 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of MCM2 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). MCM2 (arrow) was detected using the purified Pab.

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma reacted with MCM2 Antibody (C-term), which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of the MCM2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 656~685 amino acids from the C-terminal region of human MCM2
• Specificity: This antibody recognizes Human MCM2 (C-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens minichromosome maintenance complex component 2 (MCM2), transcript variant 1
• Database Link:
  Entrez Gene 4171 Human
• Background: MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
• Synonyms: BM28, CCNL1, CDCL1, Nuclear protein BM28
• Note: Molecular Weight: 101896 Da

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, DNA replication