MECP2 (N-term) Rabbit Polyclonal Antibody

CAT#: AP52648PU-N

MECP2 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of MeCP2 Antibody (N-term) in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the MeCP2 antibody detected the MeCP2 protein (arrow).

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  Formalin fixed, paraffin embedded human brain tissue stained with MeCP2 Antibody (N-term) followed by peroxidase conjugation of the secondary antibody and DAB staining.

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  Flow cytometric analysis of MDA-MB435 cells using MeCP2 Antibody (N-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide selected from the N-terminal region of Human MeCP2.
• Specificity: This antibody recognizes Human MeCP2 (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens methyl-CpG binding protein 2 (MECP2), transcript variant 2
• Database Link:
  Entrez Gene 4204 Human
• Background: DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
• Synonyms: MeCP-2 protein
• Note: Molecular Weight: 52441 Da

Reference Data

• Protein Families: Druggable Genome