MIA40 (CHCHD4) (C-term) Rabbit Polyclonal Antibody

CAT#: AP52691PU-N

MIA40 (CHCHD4) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in MDA-MB231 cell line lysates (35ug/lane) using CHCHD4 / MIA40 antibody. This demonstrates the MIA40 antibody detected the MIA40 protein (arrow).

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  Immunohistochemistry analysis in human liver tissue (formalin-fixed, paraffin-embedded) using CHCHD4 / MIA40 antibody. (C-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MIA40 antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: This antibody is suitable for immunohistochemistry on paraffin sections and Western blotting.
  The suggested dilution is:
  Immunohistochemistry on paraffin sections: 1/10-1/50.
  Western blotting: 1/100-1/500.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 108-137aa) of human CHCHD4 / MIA40.
• Specificity: This antibody detects CHCHD4 / MIA40.
• Formulation: PBS with 0.09% (W/V) Sodium Azide.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a protein A column; followed by peptide affinity purification.
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens coiled-coil-helix-coiled-coil-helix domain containing 4 (CHCHD4), transcript variant 1
• Database Link:
  Entrez Gene 131474 Human
• Background: CHCHD4 / MIA40, a component of human mitochondria, belongs to a protein family whose members share 6 highly conserved cysteine residues constituting a -CXC-CX(9)C-CX(9)C- motif in the C terminus (Hofmann et al., 2005 [PMID 16185709]).
• Synonyms: FLJ31709; MIA40
• Note: Molecular Weight: 15996 Da