MRPS12 (Center) Rabbit Polyclonal Antibody

CAT#: AP52751PU-N

MRPS12 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of MRPS12 in CEM cell line lysates (35ug/lane). This demonstrates the MRPS12 antibody detected the MRPS12 protein (arrow).

• 

  Formalin fixed and paraffin embedded human pancreas tissue stained with MRPS12 followed by peroxidase conjugation of the secondary antibody and DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 35-67 amino acids from the Central region of Human MRPS12
• Specificity: This antibody recognizes Human MRPS12 (Center).
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens mitochondrial ribosomal protein S12 (MRPS12), transcript variant 1
• Database Link:
  Entrez Gene 6183 Human
• Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5' UTR have been found for this gene; all three variants encode the same protein
• Synonyms: MRP-S12, MT-RPS12, RPMS12, RPSM12, S12mt
• Note: Molecular Weight: 15173 Da

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency