EMA (MUC1) Rabbit Polyclonal Antibody

CAT#: AP52774PU-N

EMA (MUC1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in MDA-MB435 cell line lysates (35ug/lane) using CD227 / Mucin-1 / MUC1 antibody. This demonstrates this antibody detected the MUC1 protein (arrow).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1/100-1/500.
  Enzyme immunoassay: 1/1000.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to human CD227 / Mucin-1 / MUC1 (between 1202-1231aa)
• Specificity: This antibody recognizes human CD227 / Mucin-1 / MUC1.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens mucin 1, cell surface associated (MUC1), transcript variant 2
• Database Link:
  Entrez Gene 4582 Human
• Background: The MUC1 gene is a member of the mucin family and encodes a membrane bound, glyco- sylated phosphoprotein anchored to the apical surface of many epithelia by a transmem- brane domain, with the degree of glycosylation varying with cell type. It includes a 20aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from
  20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Its overexpression, aberrant intracellular localization and changes in glycosylation have been associated with carcino- mas. Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length nature of only some has been determined.
• Synonyms: MUC-1, PEMT, Episialin, EMA, H23AG, PUM, DF3, CA 15-3
• Note: Molecular Weight: 122102 Da

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane