MYH (MUTYH) (Center) Rabbit Polyclonal Antibody

CAT#: AP52779PU-N

MYH (MUTYH) (Center) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of MUTYH (arrow) in mouse lung tissue lysates (35ug/lane) using MUTYH antibody.

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  Confocal immunofluorescent analysis of Hela cells using MUTYH antibody., followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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  Flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram) using MUTYH antibody., followed by FITC-conjugated goat-anti-rabbit secondary antibodies.

Specifications

Product Data

• Applications: FC, IF, WB
• Recommended Dilution: Flow cytometry: 1/10-1/50.
  Immunofluorescence: 1/10-1/50.
  Western blot: 1/50-1/100.
  Enzyme immunoassay: 1/1000.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the central region (between 352-381aa) of human MUTYH.
• Specificity: This antibody recognizes MUTYH.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens mutY DNA glycosylase (MUTYH), transcript variant alpha3
• Database Link:
  Entrez Gene 70603 MouseEntrez Gene 4595 Human
• Background: MYH is the eukaryotic homolog to the E. coli protein MutY. It was first identified in HeLa cells and nicks DNA replication errors, specifically A/G mismatches. MYH is involved in oxidative DNA damage repair and initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. It possesses both adenine and 2-OH-A DNA glycosylase activities.
• Synonyms: MYH, MutY homolog
• Note: Molecular Weight: 60069 Da

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Base excision repair