MYLK3 (Center) Rabbit Polyclonal Antibody

CAT#: AP52792PU-N

MYLK3 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in K562 cell line lysates (35ug/lane) using MLCK antibody. This demonstrates this antibody detected the MLCK protein (arrow).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry on Paraffin sections: 1/10-1/50.
  Western blot: 1/100-1/500.
  Enzyme immunoassay: 1/1000.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the central region (between 542-570aa) of human MLCK / MYLK3
• Specificity: This antibody recognizes human MYLK / MYLK3.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens myosin light chain kinase 3 (MYLK3), transcript variant 1
• Database Link:
  Entrez Gene 91807 Human
• Background: Phosphorylation of cardiac myosin heavy chains (like MYH7B) and light chains (like MYL2) by a kinase, such as MYLK3, potentiates the force and rate of cross-bridge recruitment in cardiac myocytes [Chan et al., 2008; PubMed 18202317].
• Synonyms: MYLK3, Putative myosin light chain kinase 3
• Note: Molecular Weight: 88393 Da

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Calcium signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Vascular smooth muscle contraction