NARS (N-term) Rabbit Polyclonal Antibody

CAT#: AP52812PU-N

NARS (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of NARS (arrow) using NARS Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NARS gene.

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  Western blot analysis of NARS Antibody (N-term) in WiDr, HepG2 cell line lysates (35ug/lane).This demonstrates the NARS antibody detected the NARS protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma reacted with NARS Antibody (N-term), which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of NARS Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  Flow cytometric analysis of WiDr cells using NARS Antibody (N-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 31-60 amino acids from the N-terminal region of human NARS
• Specificity: This antibody recognizes Human NARS (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens asparaginyl-tRNA synthetase (NARS)
• Database Link:
  Entrez Gene 4677 Human
• Background:
  Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Asparaginyl-tRNA synthetase is localized to the cytoplasm and belongs to the class II family of tRNA synthetases. The N-terminal domain represents the signature sequence for the eukaryotic asparaginyl-tRNA synthetases.
• Synonyms: ASNS, Asparaginyl tRNA synthetase
• Note: Molecular Weight: 62943 Da

Reference Data

• Protein Pathways: Aminoacyl-tRNA biosynthesis