p95 NBS1 (NBN) (C-term) Rabbit Polyclonal Antibody

CAT#: AP52817PU-N

p95 NBS1 (NBN) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Nibrin Antibody (C-term) in NCI-H460, NCI-H292 cell line lysates (35ug/lane).

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  Confocal immunofluorescent analysis of Nibrin Antibody (C-term) with Hela cell followed by Alexa Fluor®488-conjugated Goat anti-Rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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  Flow cytometric analysis of NCI-H460 cells using Nibrin Antibody (C-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunofluorescence: 1/10-1/50.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 609-638 amino acids from the C-terminal region of Human Nibrin
• Specificity: This antibody recognizes Human Nibrin (C-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens nibrin (NBN)
• Database Link:
  Entrez Gene 4683 Human
• Background: Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.
• Synonyms: NBN, NBS, NBS1, P95
• Note: Molecular Weight: 84959 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Homologous recombination