NDUFS2 (Center) Rabbit Polyclonal Antibody

CAT#: AP52844PU-N

NDUFS2 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of NDUFS2 Antibody (Center) in Y79, A375, Hela cell line and mouse brain, cerebellum tissue lysates (35ug/lane). This demonstrates the NDUFS2 antibody detected the NDUFS2 protein (arrow).

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  Immunohistochemistryanalysis in formalin fixed and paraffin embedded hepatocarcinoma reacted with NDUFS2 Antibody (Center), which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of the NDUFS2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.

• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 293~323 amino acids from the Central region of human NDUFS2
• Specificity: This antibody recognizes Human and Mouse NDUFS2 (Center).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens NADH:ubiquinone oxidoreductase core subunit S2 (NDUFS2), transcript variant 2
• Database Link:
  Entrez Gene 226646 MouseEntrez Gene 4720 Human
• Background:
  NDUFS2 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria.
• Synonyms: Complex I-49kD, CI-49kD
• Note: Molecular Weight: 52546 Da

Reference Data

• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease