NR0B2 (Center) Rabbit Polyclonal Antibody

CAT#: AP52928PU-N

NR0B2 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

NR0B2 Antibody (Center) (PEI 1/100) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NR0B2 antibody detected the NR0B2 protein (arrow).

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  NR0B2 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NR0B2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

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  Confocal immunofluorescent analysis of NR0B2 Antibody (Center) with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100 - 1/500.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Immunofluorescence: 1/10 - 1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 56-83 amino acids from the Central region of human NR0B2
• Specificity: This antibody reacts to NR0B2.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) sodium azide
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 28058 Da
• Gene Name: Homo sapiens nuclear receptor subfamily 0 group B member 2 (NR0B2)
• Database Link:
  Entrez Gene 8431 Human
• Background: The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function.
• Synonyms: SHP

Reference Data

• Protein Families: Druggable Genome, Transcription Factors