NSUN2 (Center) Rabbit Polyclonal Antibody

CAT#: AP52942PU-N

NSUN2 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

NSUN2 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the NSUN2 antibody detected the NSUN2 protein (arrow).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1/1000.
  Western blot: 1/100 - 1/500.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 426-455 amino acids from the Central region of human NSUN2
• Specificity: This antibody reacts to NSUN2.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) sodium azide
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 86471 Da
• Gene Name: Homo sapiens NOP2/Sun RNA methyltransferase family member 2 (NSUN2), transcript variant 2
• Database Link:
  Entrez Gene 28114 MouseEntrez Gene 54888 Human
• Background: Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA) (Brzezicha et al., 2006 [PubMed 17071714])
• Synonyms: SAKI, TRM4, tRNA (cytosine-5-)-methyltransferase NSUN2