NUDT15 (C-term) Rabbit Polyclonal Antibody

CAT#: AP52959PU-N

NUDT15 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in HepG2 cell line lysates (35ug/lane) using NUDT15 antibody. (C-term). This demonstrates the NUDT15 antibody detected the NUDT15 protein (arrow).

• 

  Western blot analysis in mouse bladder tissue lysates (35ug/lane) using NUDT15 antibody. (C-term). This demonstrates the NUDT15 antibody detected the NUDT15 protein (arrow).

• 

  Immunohistochemistry analysis in human breast tissuee (Formalin-fixed, Paraffin-embedded) using NUDT15 antibody. (C-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NUDT15 antibody for IHC. Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution:

  Flow cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin sections: 1/50-1/100.
  Western blot: 1/1000.
  Enzyme immunoassay: 1/1000.

• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 89-118aa) of human NUDT15.
• Specificity: This antibody recognizes NUDT15.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens nudix hydrolase 15 (NUDT15), transcript variant 1
• Database Link:
  Entrez Gene 55270 Human
• Background: Mediates the hydrolysis of some nucleoside diphosphate derivatives. Can degrade 8-oxo-dGTP in vitro, suggesting that it may remove an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA and the nucleotide pool, thereby preventing misincorporation of 8-oxo-dGTP into DNA thus preventing A:T to C:G transversions. Its substrate specificity in vivo however remains unclear (By similarity). May have a role in DNA synthesis and cell cycle progression throught the interaction with PCNA.
• Synonyms: MTH2, MutT homolog 2, Nudix motif 15
• Note: Molecular Weight: 18609 Da