NUP153 (N-term) Rabbit Polyclonal Antibody

CAT#: AP52970PU-N

NUP153 (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of NUP153 Antibody (N-term) in Hela cell line lysates (35ug/lane). NUP153 (arrow) was detected using the purified Pab.

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  Flow cytometry analysis of 293 cells using NUP153 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 65~94 amino acids from the N-terminal region of Human NUP153
• Specificity: This antibody recognizes Human NUP153 (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens nucleoporin 153 (NUP153), transcript variant 2
• Database Link:
  Entrez Gene 9972 Human
• Background: Nuclear pore complexes are extremely elaborate structures that mediate the regulated movement of macromolecules between the nucleus and cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are pore complex-specific glycoproteins characterized by cytoplasmically oriented O-linked N-acetylglucosamine residues and numerous repeats of the pentapeptide sequence XFXFG. This protein has three distinct domains: a N-terminal region within which a pore targeting domain has been identified, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats.
• Synonyms: Nucleoporin Nup153
• Note: Molecular Weight: 153938 Da

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency