PAX5 (Center) Rabbit Polyclonal Antibody

CAT#: AP53179PU-N

PAX5 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

PAX5 Antibody (Center) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the PAX5 antibody detected the PAX5 protein (arrow).

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  PAX5 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PAX5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

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  PAX5 Antibody (Center) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100 - 1/500.
  Flow Cytometry: 1/10 - 1/50.
  Immunohistochemistry on paraffin sections: 1/10 - 1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 185-215 amino acids from the Central region of human PAX5
• Specificity: This antibody reacts to PAX5.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) sodium azide
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 42149 Da
• Gene Name: Homo sapiens paired box 5 (PAX5), transcript variant 1
• Database Link:
  Entrez Gene 5079 Human
• Background: This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternatively spliced transcript variants encoding different isoforms have been described but their biological validity has not been determined.
• Synonyms: Pax-5, BSAP

Reference Data

• Protein Families: Transcription Factors