PCBD1 (Center) Rabbit Polyclonal Antibody

CAT#: AP53190PU-N

PCBD1 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of PCBD1 Antibody (Center) in mouse liver tissue lysates (35ug/lane). PCBD1 (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with PCBD1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  PCBD1 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100 - 1/500.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Flow Cytometry: 1/10 - 1/50.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 27~56 amino acids from the Center region of human PCBD1
• Specificity: This antibody reacts to PCBD1.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) sodium azide
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 12000 Da
• Gene Name: Homo sapiens pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (PCBD1), transcript variant 1
• Database Link:
  Entrez Gene 13180 MouseEntrez Gene 5092 Human
• Background: PCBD1 encodes pterin-4 alpha-carbinolamine dehydratase, an enzyme involved in phenylalanine hydroxylation. A deficiency of this enzyme leads to hyperphenylalaninemia. The enzyme regulates the homodimerization of the transcription factor hepatocyte nuclear factor 1 (HNF1).
• Synonyms: DCOH, PCBD, PHS, Pterin carbinolamine dehydratase

Reference Data

• Protein Families: Druggable Genome