PCDHGC3 (Center) Rabbit Polyclonal Antibody

CAT#: AP53216PU-N

PCDHGC3 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in Jurkat cell line lysates (35ug/lane) using PCDHGC3 antibody. This demonstrates the PCDHGC3 antibody detected the PCDHGC3 protein (arrow).

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  Immunohistochemistry analysis human brain tissue (Formalin-fixed, Paraffin-embedded) using PCDHGC3 antibody., followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PCDHGC3 antibody for IHC; Clinical relevance has not been evaluated.

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  Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram) using PCDHGC3 antibody., followed by FITC-conjugated goat-anti-rabbit secondary antibodies.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution:

  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.
  Western blot: 1/1000.
  

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 511-539 amino acids from the to the central region of human PCDHGC3.
• Specificity: This antibody recognizes Human PCDHGC3.
  Other species not tested.
  Cellular Location:
  Cell membrane; Single-pass type I membrane protein.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens protocadherin gamma subfamily C, 3 (PCDHGC3), transcript variant 1
• Database Link:
  Entrez Gene 5098 Human
• Background: The PCDHGC3 gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.
• Synonyms: Protocadherin gamma-C3, PCDH-gamma-C3, Protocadherin-2, Protocadherin 2, PCDH2, Protocadherin-43, Protocadherin 43, PC-43, PC43
• Note: Molecular Weight: 101077 Da

Reference Data

• Protein Families: Transmembrane