PIK3R5 (C-term) Rabbit Polyclonal Antibody

CAT#: AP53306PU-N

PIK3R5 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in WiDr cell line lysates (35ug/lane) using PIK3R5 antibody. (C-term). This demonstrates the PIK3R5 antibody detected the PIK3R5 protein (arrow).

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  Immunohistochemistry analysis in human tonsil tissue (Formalin-fixed, Paraffin-embedded) using PIK3R5 antibody. (C-term), followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of PIK3R5 antibody for IHC. Clinical relevance has not been evaluated.

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  Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram) using PIK3R5 antibody. (C-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodies.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution:

  Flow cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin sections: 1/50-1/100.
  Western blot: 1/100-1/500.
  Enzyme immunoassay: 1/1000.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 770-800aa) of human PIK3R5.
• Specificity: This antibody recognizes PIK3R5 at C-term.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens phosphoinositide-3-kinase regulatory subunit 5 (PIK3R5), transcript variant 1
• Database Link:
  Entrez Gene 23533 Human
• Background: Receptor-regulated class I phosphoinositide 3-kinases (PI3Ks) phosphorylate the membrane lipid phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to PtdIns(3,4,5)P3, which in turn recruits and activates cytosolic effectors involved in proliferation, survival, or chemotaxis. PIK3R5 is a PI3K regulatory subunit [Brock et al., 2003; PubMed 12507995].
• Synonyms: p101-PI3K, FOAP-2, PI3-kinase regulatory subunit 5, PtdIns-3-kinase p101
• Note: Molecular Weight: 55kd Isoform 2. 97348 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Insulin signaling pathway, Jak-STAT signaling pathway, Leukocyte transendothelial migration, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Phosphatidylinositol signaling system, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, VEGF signaling pathway