Phospholamban (PLN) (Thr7) Rabbit Polyclonal Antibody

CAT#: AP53332PU-N

Phospholamban (PLN) (Thr7) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of PLB-Thr17 in Jurkat cell line lysates (35ug/lane). PLB (arrow) was detected using the purified Pab.

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue using PLB-Thr17 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PLB-T17 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

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  Flow cytometric analysis of Jurkat cells using PLB-Thr17 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: Western blot: 1/1000.
  Flow cytometry: 1/10-50.
  Immunohistochemistry on Paraffin sections: 1/50-1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide selected between 1-30 amino of Human Phospholamban
• Specificity: This antibody recognizes Phospholamban (Thr17)
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: phospholamban
• Database Link:
  Entrez Gene 5350 Human
  UniProt ID P26678
• Background: PLB is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer.
• Synonyms: Cardiac phospholamban, PLB, PLN
• Note: Molecular Weight: 6109 Da