PLOD1 (N-term) Rabbit Polyclonal Antibody

CAT#: AP53354PU-N

PLOD1 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of PLOD1 / PLOD Antibody (N-term) in U251 cell line lysates (35ug/lane). This demonstrates the PLOD1 antibody detected the PLOD1 protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue reacted with PLOD1 / PLOD Antibody (N-term), which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of PLOD1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  Flow cytometric analysis of U251 cells using PLOD1 / PLOD Antibody (N-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 73-102 amino acids from the N-terminal region of human PLOD1 / PLOD
• Specificity: This antibody recognizes Human PLOD1 / PLOD (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1), transcript variant 2
• Database Link:
  Entrez Gene 5351 Human
• Background: Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity.
• Synonyms: Lysyl hydroxylase 1, LLH
• Note: Molecular Weight: 83550 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Lysine degradation