PON2 (N-term) Rabbit Polyclonal Antibody

CAT#: AP53391PU-N

PON2 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of PON2 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). This demonstrates the PON2 antibody detected the PON2 protein (arrow).

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  Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue reacted with PON2 Antibody (N-term), which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of PON2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  Flow cytometric analysis of K562 cells using PON2 Antibody (N-term) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 78-107 amino acids from the N-terminal region of human PON2.
• Specificity: This antibody recognizes Human PON2 (N-term).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens paraoxonase 2 (PON2), transcript variant 1
• Database Link:
  Entrez Gene 5445 Human
• Background: This gene encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described.
• Synonyms: PON-2, PON 2, Aromatic esterase 2, A-esterase 2
• Note: Molecular Weight: 39398 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways