AMPK gamma 1 (PRKAG1) (N-term) Rabbit Polyclonal Antibody

CAT#: AP53443PU-N

AMPK gamma 1 (PRKAG1) (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

PRKAG1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PRKAG1 antibody detected the PRKAG1 protein (arrow).

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  PRKAG1 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the PRKAG1 antibody detected the PRKAG1 protein (arrow).

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  Western blot analysis of PRKAG1 (arrow) using rabbit polyclonal PRKAG1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRKAG1 gene.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100 - 1/500.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 5-33 amino acids from the N-terminal region of human PRKAG1
• Specificity: This antibody reacts to PRKAG1.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% (W/V) sodium azide
• Concentration: lot specific
• Purification: Affinity chromatography on Protein A
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 37579 Da
• Gene Name: Homo sapiens protein kinase AMP-activated non-catalytic subunit gamma 1 (PRKAG1), transcript variant 3
• Database Link:
  Entrez Gene 19082 MouseEntrez Gene 5571 Human
• Background: The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Synonyms: PRKAG1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway