PYCR1 (C-term) Rabbit Polyclonal Antibody

CAT#: AP53528PU-N

PYCR1 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of PYCR1 (arrow) in K562, WiDr, HepG2 cell line lysates (35ug/lane) using PYCR1 antibody. (C-term).

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  Immunohistochemistry analysis in colon carcinoma (Formalin-fixed, Paraffin-embedded) using PYCR1 antibody. (C-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PYCR1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram) using PYCR1 antibody. (C-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodies.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution:

  Flow cytometry: 1/10-1/50.
  Immunohistochemistry on Paraffin sections: 1/50-1/100.
  Western blot: 1/100-1/500.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 290-319aa) of human PYCR1.
• Specificity: This antibody recognizes PYCR1 at C-term.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens pyrroline-5-carboxylate reductase 1 (PYCR1), transcript variant 1
• Database Link:
  Entrez Gene 5831 Human
• Background: The PYCR1 gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. The P5C reductase 1 may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion.
• Synonyms: P5C reductase 1, P5CR 1
• Note: Molecular Weight: 33361 Da

Reference Data

• Protein Pathways: Arginine and proline metabolism, Metabolic pathways