RAD17 (Center) Rabbit Polyclonal Antibody

CAT#: AP53558PU-N

RAD17 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of RAD17 Antibody in A549 cell line lysates (35ug/lane). This demonstrates the RAD17 antibody detected the RAD17 protein (arrow).

• 

  Flow cytometric analysis of A549 cells using RAD17 Antibody (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 225-254 amino acids from the Central region of Human RAD17
• Specificity: This antibody recognizes Human RAD17 (Center).
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens RAD17 checkpoint clamp loader component (RAD17), transcript variant 8
• Database Link:
  Entrez Gene 5884 Human
• Background: The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.
• Synonyms: R24L
• Note: Molecular Weight: 77055 Da

Reference Data

• Protein Families: Druggable Genome