RECK (Center) Rabbit Polyclonal Antibody

CAT#: AP53626PU-N

RECK (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of RECK / ST15 Antibody in HepG2 cell line lysates (35ug/lane). RECK (arrow) was detected using the purified Pab.

• 

  Formalin-fixed and paraffin-embedded human brain tissue reacted with RECK / ST15 Antibody which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

• 

  Flow cytometric analysis of k562 cells using RECK / ST15 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution:

  ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-/50.
  Immunohistochemistry on Paraffin Sections: 1/50-1/100.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 436~465 amino acids from the Center region of Human RECK.
• Specificity: This antibody recognizes Human RECK / ST15 (Center).
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens reversion inducing cysteine rich protein with kazal motifs (RECK), transcript variant 1
• Database Link:
  Entrez Gene 8434 Human
• Background:
  RECK is a cysteine-rich, extracellular protein with protease inhibitor-like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane-anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase-9, a key enzyme involved in tumor invasion and metastasis.
• Synonyms: hRECK; ST15
• Note: Molecular Weight: 106457 Da

Reference Data

• Protein Families: Transmembrane