RPL31 (Center) Rabbit Polyclonal Antibody

CAT#: AP53716PU-N

RPL31 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in HeLa cell line lysates (35 ug/lane) using RPL31 Antibody (Center). This demonstrates the RPL31 antibody detected the RPL31 protein (arrow).

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  Immunohistochemistry analysis in Formalin Fixed, Paraffin Embedded Human Testis using RPL31 Antibody (Center). followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of RPL31 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

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  Flow Cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram) using RPL31 Antibody (Center). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide selected from the Central region of Human RPL31.
• Specificity: Recognizes RPL31 (Center).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column followed by peptide Affinity purification
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens ribosomal protein L31 (RPL31), transcript variant 2
• Database Link:
  Entrez Gene 6160 Human
  UniProt ID P62899
• Background: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL31 is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L31E family of ribosomal proteins. It is located in the cytoplasm.
• Synonyms: 60S ribosomal protein L31
• Note: Molecular Weight: 14463 Da

Reference Data

• Protein Pathways: Ribosome