SDHD (N-term) Rabbit Polyclonal Antibody

CAT#: AP53823PU-N

SDHD (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of SDHD Antibody (N-term) in Hela cell line lysates (35ug/lane). This demonstrates the SDHD antibody detected the SDHD protein (arrow).

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  Immunohistochemistry analysis in Formalin Fixed, Paraffin Embedded Human kidney tissue stained with SDHD Antibody (N-term) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of SDHD Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  Confocal Immunofluorescent analysis of SDHD Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated Goat anti-Rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/100-1/500.
  Immunofluorescence: 1/10-1/50.
  Immunohistochemistry: 1/10-1/50.
  Flow Cytometry: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 20-50 amino acids from the N-terminal region of Human SDHD (NP_002993.1)
• Specificity: Recognizes SDHD (N-term).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column followed by peptide Affinity purification
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens succinate dehydrogenase complex, subunit D, integral membrane protein (SDHD), transcript variant 1
• Database Link:
  Entrez Gene 6392 Human
• Background: Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The subunit D protein is one of two integral membrane proteins anchoring the complex to the matrix side of the membrane. Mutations in SDHD have been linked to hereditary paraganglioma.
• Synonyms: SDH4, CybS, QPs3, CII-4, Succinate dehydrogenase complex subunit D
• Note: Molecular Weight: 17043 Da

Reference Data

• Protein Pathways: Alzheimer's disease, Citrate cycle (TCA cycle), Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease