SEC62 (N-term) Rabbit Polyclonal Antibody

CAT#: AP53834PU-N

SEC62 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of SEC62 Antibody (N-term) in ZR-75-1 cell line lysates (35ug/lane). This demonstrates the SEC62 antibody detected the SEC62 protein (arrow).

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  Immunohistochemistry analysis in Formalin Fixed, Paraffin Embedded Human brain tissue stained with SEC62 antibody (C-term) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of SEC62 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/100-1/500.
  Immunohistochemistry: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of Human SEC62
• Specificity: Recognizes SEC62 (N-term).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column followed by peptide Affinity purification
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens SEC62 homolog, preprotein translocation factor (SEC62)
• Database Link:
  Entrez Gene 7095 Human
• Background: The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. The protein encoded by this gene and SEC63 protein are found to be associated with ribosome-free SEC61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. The encoded protein is an integral membrane protein located in the rough ER.
• Synonyms: Dtrp1; FLJ32803; hTP-1; HTP1; TLOC1; TP-1
• Note: Molecular Weight: 45862 Da

Reference Data

• Protein Families: Druggable Genome, Transmembrane