SESN3 (Center) Rabbit Polyclonal Antibody

CAT#: AP53867PU-N

SESN3 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis using Sestrin-3 Antibody (Center Y335) in A549 cell line lysates (35ug/lane). This demonstrates the Sestrin-3 antibody detected the Sestrin-3 protein (arrow).

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  Immunohistochemistry analysis in Formalin Fixed, Paraffin Embedded Human pancreas tissue stained with antibody (Center Y335) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of Sestrin-3 Antibody (Center Y335) for immunohistochemistry. Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/100-1/500.
  Immunohistochemistry: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 319-347 amino acids from the Central region of Human Sestrin-3
• Specificity: Recognizes Sestrin-3 (Center Y335).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column followed by peptide Affinity purification
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens sestrin 3 (SESN3), transcript variant 1
• Database Link:
  Entrez Gene 143686 Human
• Background: Sestrins form a small family of redox enzymes which regulate accumulation of Reactive Oxygen Species. They protect cells and their DNA against oxidative damage and regulate cell growth and viability. These genes are often deregulated in cancers and their inactivation accelerates the growth of model tumors in mice.
• Synonyms: SEST3
• Note: Molecular Weight: 57291 Da

Reference Data

• Protein Pathways: p53 signaling pathway