SFRP1 (N-term) Rabbit Polyclonal Antibody

CAT#: AP53873PU-N

SFRP1 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of SFRP1 Antibody (N-term) in K562 cell line lysates (35ug/lane). SFRP1 (arrow) was detected using the purified Pab.

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  Formalin-Fixed and paraffin-Embedded Human testis tissue reacted with SFRP1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  Flow Cytometry analysis of K562 cells using SFRP1 Antibody (N-term) (Bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
  Immunohistochemistry: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 33~63 amino acids from the N-terminal region of Human SFRP1.
• Specificity: Recognizes SFRP1 (N-term).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A column followed by peptide Affinity purification
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens secreted frizzled related protein 1 (SFRP1)
• Database Link:
  Entrez Gene 6422 Human
• Background: SFRP1 encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer.
• Synonyms: SFRP-1, FRP-1, FRP1, FRP, SARP2, SARP-2
• Note: Molecular Weight: 35386 Da

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway, Transmembrane
• Protein Pathways: Wnt signaling pathway