SPECC1L (Center) Rabbit Polyclonal Antibody

CAT#: AP54013PU-N

SPECC1L (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of SPECC1 Antibody (Center) in HL-60 cell line lysates (35ug/lane). This demonstrates the SPECC1 antibody detected the SPECC1 protein (arrow).

• 

  Western blot analysis of SPECC1 Antibody (Center) in Mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the SPECC1 antibody detected the SPECC1 protein (arrow).

• 

  Formalin fixed, paraffin embedded human brain tissue stained with SPECC1 Antibody (Center) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of SPECC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

• 

  Confocal immunofluorescent analysis of SPECC1 Antibody (Center) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

• 

  Flow cytometric analysis of HL-60 cells using SPECC1 Antibody (Center) (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Show all 5 images

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: Western Blot: 1/1000.
  Flow Cytometry: 1/10-1/50.
  Immunofluorescence: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 705-733 amino acids from the central region of Human SPECC1-like protein.
• Specificity: This antibody recognizes Human and Mouse SPECC1L (Center).
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens sperm antigen with calponin homology and coiled-coil domains 1 like (SPECC1L), transcript variant 2
• Database Link:
  Entrez Gene 23384 Human
• Synonyms: CYTSA,Cytospin-A,KIAA0376,NY-REN-22,SPECC1-like protein
• Note: Molecular Weight: 124602 Da