SPG7 (Center) Rabbit Polyclonal Antibody

CAT#: AP54017PU-N

SPG7 (Center) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis in 293 cell line lysates (35ug/lane) using Paraplegin / SPG7 antibody. This demonstrates the SPG7 antibody detected the SPG7 protein (arrow).

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  Immunohistochemistry analysis in human brain tissue (Formalin-fixed, Paraffin-embedded) using Paraplegin / SPG7 antibody., followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of SPG7 antibody for IHC; Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution:

  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
  Western blot: ~1/1000.
  Enzyme immunoassay: 1/1000.

• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated corresponding to the central region (between 115-141aa) of Human Paraplegin / SPG7
• Specificity: This antibody recognizes Human Paraplegin / SPG7.
  Other species not tested.
• Formulation: PBS with 0.09% (W/V) Sodium azide
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Purified through a Protein A column followed by peptide affinity purification
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 88235 Da (calculated)
• Gene Name: Homo sapiens SPG7, paraplegin matrix AAA peptidase subunit (SPG7), transcript variant 1
• Database Link:
  Entrez Gene 6687 Human
• Background: The SPG7 gene encodes a nuclear-encoded mitochondrial metalloprotease protein that is a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Two transcript variants encoding distinct isoforms have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 7.
• Synonyms: Spastic paraplegia 7 protein, CAR, CMAR, PGN

Reference Data

• Protein Families: Protease, Transmembrane